Different Modes of Synaptic Vesicle Recycling un Synaptophysin-pHluorin Transgenic Mouse Motor Nerve Terminals
In the present work, a new tool for studying synaptic vesicle exo- and endocytosis in real time has been developed (the SypHy mouse). By combining live imaging, electrophysiological recordings and immunostaining in SypHy mice we have investigated the functional and structural organization of a vertebrate model synapse, the neuromuscular junction (NMJ). The adult vertebrate NMJ is a highly reliable synapse that must exocytose a large number of vesicles with each stimulus to guarantee effective excitation of the postsynaptic muscle fiber. To maintain effective synaptic transmission, the motor nerve terminal must be able to reuse vesicles by subsequent endocytosis. Diverse modes ...